Prostate Specific Antigen Antikörper

Produktnummer ABIN3026028

Der Maus Monoklonal Anti-Prostate Specific Antigen-Antikörper wurde für IHC (p), IF und FACS validiert. Er ist geeignet, Prostate Specific Antigen in Proben von Human zu detektieren.

Kurzübersicht für Prostate Specific Antigen Antikörper (ABIN3026028)

Target: Prostate Specific Antigen (PSA)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Prostate Specific Antigen Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: A67-B-E3-1A7

Produktdetails anti-Prostate Specific Antigen Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: PSA (p30) from human sperm plasma was used as the immunogen for the Prostate Specific Antigen antibody cocktail.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Prostate Specific Antigen antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Prostate Specific Antigen antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu Prostate Specific Antigen

Target: Prostate Specific Antigen (PSA)

Andere Bezeichnung: Prostate Specific Antigen

Hintergrund: Recognizes a single protein of 33-34  kDa, identified as the prostate specific antigen (PSA). This mAb is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia.

Pathways: Komplementsystem