CD44 Antikörper

Produktnummer ABIN3025607

Dieser Maus Monoklonal Antikörper detektiert spezifisch CD44 in FACS, WB, IF, IHC (p) und Func. Es zeigt Reaktivität gegenüber Proben von Human, Pavian und Green Monkey.

Kurzübersicht für CD44 Antikörper (ABIN3025607)

Target: CD44

Reaktivität: Human, Pavian, Green Monkey

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD44 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)

Klon: SPM544

Produktdetails anti-CD44 Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: Stimulated human leukocytes were used as the immunogen for this anti-CD44 antibody.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: The optimal dilution of the anti-CD44 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Functional Studies: Order Ab without BSA/azide,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the anti-CD44 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu CD44

Target: CD44

Andere Bezeichnung: CD44

Hintergrund: Recognizes a cell surface glycoprotein of 80-95  kDa (CD44) on lymphocytes, monocytes, and granulocytes. Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95  kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.

Pathways: Glycosaminoglycan Metabolic Process, Autophagie, Negative Regulation of intrinsic apoptotic Signaling