PODXL Antikörper (Extracellular Domain, Internal Region)

Produktnummer ABIN3025409

Dieses Maus Monoklonal-Antikörper erkennt spezifisch PODXL in FACS, IF und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Ratte und Kaninchen.

Kurzübersicht für PODXL Antikörper (Extracellular Domain, Internal Region) (ABIN3025409)

Target: PODXL (Podocalyxin-Like (PODXL))

Reaktivität: Human, Ratte, Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PODXL Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 2A4

Produktdetails anti-PODXL Antikörper

Bindungsspezifität: Extracellular Domain, Internal Region

Aufreinigung: PEG precipitation

Immunogen: A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human PODXL was used as the immunogen for the Podocalyxin antibody.

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Podocalyxin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Podocalyxin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu PODXL

Target: PODXL (Podocalyxin-Like (PODXL))

Andere Bezeichnung: Podocalyxin

Hintergrund: Podocalyxin/PODXL is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.

Pathways: Tube Formation