PODXL Antikörper (Extracellular Domain)

Produktnummer ABIN3025406

Dieses Maus Monoklonal-Antikörper erkennt spezifisch PODXL in WB, IP, FACS, IF und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Ratte und Kaninchen.

Kurzübersicht für PODXL Antikörper (Extracellular Domain) (ABIN3025406)

Target: PODXL (Podocalyxin-Like (PODXL))

Reaktivität: Human, Ratte, Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PODXL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 3D3

Produktdetails anti-PODXL Antikörper

Bindungsspezifität: Extracellular Domain

Aufreinigung: Protein G affinity chromatography

Immunogen: A human recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain was used as the immunogen for the Podocalyxin antibody.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Podocalyxin antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IP: 1-2 μg/500 μg protein,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Podocalyxin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu PODXL

Target: PODXL (Podocalyxin-Like (PODXL))

Andere Bezeichnung: Podocalyxin

Hintergrund: Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.

Gen-ID: 5420

Pathways: Tube Formation