PLAP Antikörper

Produktnummer ABIN3025398

Der Maus Monoklonal Anti-PLAP-Antikörper wurde für FACS, IF und IHC (fro) validiert. Er ist geeignet, PLAP in Proben von Human zu detektieren.

Kurzübersicht für PLAP Antikörper (ABIN3025398)

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PLAP Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: ALPP-238

Produktdetails anti-PLAP Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: Recombinant full-length human ALPP protein was used as the immunogen for the placental Alkaline Phosphatase antibody.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the placental Alkaline Phosphatase antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (Frozen): 1-2 μg/mL for 30 min at RT

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the placental Alkaline Phosphatase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu PLAP

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Andere Bezeichnung: Placental Alkaline Phosphatase

Hintergrund: PLAP is a tissue specific, trophoblast-derived, 70  kDa, glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2+ metallo glycoprotein that catalyzes the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. It is present in the placenta and serum of pregnant women and in high frequency in gynecological and testicular cancers and in lower frequency in other tumors. The three tissue-specific AP's in humans, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98 % homologous. Non-tissue specific AP is found in kidney, liver and bone. This mAb binds equally well to all common allelic variants (S, F, FS and I) of PLAP as to AP from normal human testis. This mAb can be used both as coating as well as tracer antibody in the same ELISA to detect PLAP in serum of S, F, FS and I phenotypes.