HSP27 Antikörper

Produktnummer ABIN3025019

Dieser Maus Monoklonal Antikörper detektiert spezifisch HSP27 in WB, IF, FACS und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für HSP27 Antikörper (ABIN3025019)

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HSP27 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Güteklasse: Carrier-free

Klon: SPM252

Produktdetails anti-HSP27 Antikörper

Verwendungszweck: HSP27 Antibody (azide and preservative free)

Produktmerkmale: It recognizes a 24-27 kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced 'p29' and '24K' protein. About 50 % of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.

Aufreinigung: Protein G affinity chromatography

Immunogen: Partially purified HSP27 protein from the human breast cancer cell line MCF-7 was used as the immunogen for this HSP27 antibody.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: The optimal dilution of the HSP27 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the HSP27 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Andere Bezeichnung: HSP27

Hintergrund: It recognizes a 24-27 kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced 'p29' and '24K' protein. About 50 % of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.

UniProt: P04792

Pathways: MAPK Signalweg, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling