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Keratin 5/8 Antikörper

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Keratin 5/8 in WB, FACS, IF und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus, Schwein, Ratte, Hund, Hamster, Rind (Kuh), Schaf und Frettchen.
Produktnummer ABIN3025013

Kurzübersicht für Keratin 5/8 Antikörper (ABIN3025013)

Target

Keratin 5/8 (KRT5/8)

Reaktivität

  • 23
  • 16
  • 15
  • 13
  • 13
  • 13
  • 12
  • 10
  • 9
  • 3
  • 2
  • 2
Human, Maus, Schwein, Ratte, Hund, Hamster, Rind (Kuh), Schaf, Frettchen

Wirt

  • 23
Maus

Klonalität

  • 23
Monoklonal

Konjugat

  • 9
  • 4
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser Keratin 5/8 Antikörper ist unkonjugiert

Applikation

  • 16
  • 15
  • 14
  • 12
  • 8
  • 8
  • 5
  • 4
  • 3
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon

SPM268
  • Keine Kreuzreaktivität

    Huhn, Xenopus laevis

    Produktmerkmale

    It reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.

    Aufreinigung

    Protein G affinity chromatography

    Immunogen

    A cytoskeletal preparation from HeLa cells was used as the immunogen for the Cytokeratin 5/8 antibody.

    Isotyp

    IgG1 kappa
  • Applikationshinweise

    Optimal dilution of the Cytokeratin 5/8 antibody should be determined by the researcher.

    1. For staining of formalin-fixed tissues, digest sections with Trypsin at 1 mg/mL PBS, 15 min at RT
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    1 mg/mL

    Buffer

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    Konservierungsmittel

    Azide free

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store the Cytokeratin 5/8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target

    Keratin 5/8 (KRT5/8)

    Andere Bezeichnung

    Cytokeratin 5/8

    Hintergrund

    It reacts with keratin 5 (58  kDa) and keratin 8 (52.5  kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
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