GZMB Antikörper (AA 73-187)

Produktnummer ABIN3024894

Der Kaninchen Polyklonal Anti-GZMB-Antikörper wurde für WB, FACS, IF und IHC (p) validiert. Er ist geeignet, GZMB in Proben von Human zu detektieren.

Kurzübersicht für GZMB Antikörper (AA 73-187) (ABIN3024894)

Target: GZMB (Granzyme B (GZMB))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GZMB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-GZMB Antikörper

Bindungsspezifität: AA 73-187

Aufreinigung: Protein A affinity chromatography

Immunogen: A recombinant fragment (amino acids 73-187) from the human protein was used as the immunogen for the Granzyme B antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Granzyme B antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Granzyme B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu GZMB

Target: GZMB (Granzyme B (GZMB))

Andere Bezeichnung: Granzyme B

Hintergrund: Granzyme B is a member of the granule serine protease family stored specifically in NK cells or cytotoxic T cells. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'non-self' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. Granzyme B is crucial for the rapid induction of target cell apoptosis by CTLs in the cell-mediated immune response. Granzyme B is useful as a marker in the identification of NK/T-cell lymphomas. High percentages of cytotoxic T-cells have been shown to be an unfavorable prognostic indicator in Hodgkin's Disease.

Pathways: Apoptose, Caspase Kaskade in der Apoptose