CD74 Antikörper

Produktnummer ABIN3024478

Dieses Anti-CD74-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD74 in FACS, WB, IF und IHC (p). Geeignet für Human und Maus.

Kurzübersicht für CD74 Antikörper (ABIN3024478)

Target: CD74 (HLA-DR-gamma (CD74))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD74 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: SPM523

Produktdetails anti-CD74 Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: SU-DHL-4 lymphoma cells were used as the immunogen for this anti-CD74 antibody.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: The optimal dilution of the anti-CD74 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL, Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 minutes at RT (1),Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the anti-CD74 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu CD74

Target: CD74 (HLA-DR-gamma (CD74))

Andere Bezeichnung: CD74

Hintergrund: CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints