beta Catenin Antikörper

Produktnummer ABIN3024225

Der Kaninchen Polyklonal Anti-beta Catenin-Antikörper wurde für WB, FACS, IF und IHC (p) validiert. Er ist geeignet, beta Catenin in Proben von Human und Maus zu detektieren.

Kurzübersicht für beta Catenin Antikörper (ABIN3024225)

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser beta Catenin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-beta Catenin Antikörper

Aufreinigung: Protein A affinity chromatography

Immunogen: A synthetic peptide from the middle of beta-Catenin / p120 protein was used as the immunogen.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 minutes at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the beta-Catenin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu beta Catenin

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Andere Bezeichnung: beta-Catenin

Hintergrund: Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, it is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution throughout the cytoplasm results in a diffuse cytoplasmic pattern. Staining with beta-catenin antibody and E-cadherin antibody helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.

Gen-ID: 1499

Pathways: Peptide Hormone Metabolism