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CLIP Antikörper (AA 25-39)

CLIP Reaktivität: Human FACS, IF, IHC (p), ELISA Wirt: Maus Monoclonal ADCT1-1 unconjugated
Produktnummer ABIN3024113
  • Target Alle CLIP Produkte
    CLIP (Corticotropin-Like Intermediate Peptide (CLIP))
    Bindungsspezifität
    • 4
    • 3
    • 2
    AA 25-39
    Reaktivität
    • 11
    • 4
    • 2
    Human
    Wirt
    • 7
    • 6
    Maus
    Klonalität
    • 8
    • 5
    Monoklonal
    Konjugat
    • 11
    • 1
    • 1
    Dieser CLIP Antikörper ist unkonjugiert
    Applikation
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA
    Aufreinigung
    Protein G affinity chromatography
    Immunogen
    Amino acids 25-39 (NGAEDESAEAFPLEF) of Adrenocorticotropic hormone were used as the immunogen for this ACTH antibody.
    Klon
    ADCT1-1
    Isotyp
    IgG1
  • Applikationshinweise
    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the ACTH antibody to be titered up or down for optimal performance.

    1. Staining of FFPE tissue requires boiling sections in 10  mM Citrate Buffer,  pH 6, for 10-20 min followed by cooling at RT for 20 min.\. ELISA: order BSA free format for coating,FACS: 0.5-1 μg/10^6 cells,IF: 1-2 μg/mL,IHC (FFPE):0.5-1 μg/mL

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1 mg/mL
    Buffer
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    Konservierungsmittel
    Azide free
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store the ACTH antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    CLIP (Corticotropin-Like Intermediate Peptide (CLIP))
    Andere Bezeichnung
    ACTH / CLIP (CLIP Produkte)
    Hintergrund
    ACTH (or Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This mAb is specific to CLIP (aa 25-39) and does not react with Synacthen (aa 1-24). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.
    Gen-ID
    5443
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