GFM1 Antikörper
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- Target Alle GFM1 Antikörper anzeigen
- GFM1 (G-Elongation Factor, Mitochondrial 1 (GFM1))
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser GFM1 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Keine Kreuzreaktivität
- Maus, Ratte (Rattus)
- Produktmerkmale
- This mAb recognizes a 60 kDa antigen associated with the mitochondria in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the mitochondria in human cells only. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.
- Aufreinigung
- Protein G affinity chromatography
- Immunogen
- A semi-purified mitochondrial preparation was used as the immunogen for the anti-Mitochondrial antibody.
- Klon
- AE-1
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product GFM1 Primärantikörper
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- Applikationshinweise
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Optimal dilution of the anti-Mitochondrial antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. ELISA: order Ab without BSA,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.25-0.5 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1 mg/mL
- Buffer
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- Konservierungsmittel
- Azide free
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store the anti-Mitochondrial antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Target
- GFM1 (G-Elongation Factor, Mitochondrial 1 (GFM1))
- Andere Bezeichnung
- Mitochondrial (GFM1 Produkte)
- Synonyme
- COXPD1 antikoerper, EFG antikoerper, EFG1 antikoerper, EFGM antikoerper, EGF1 antikoerper, GFM antikoerper, hEFG1 antikoerper, AW545374 antikoerper, D3Wsu133e antikoerper, Gfm antikoerper, EF-G antikoerper, Efg antikoerper, G elongation factor mitochondrial 1 antikoerper, G elongation factor, mitochondrial 1 antikoerper, GFM1 antikoerper, Gfm1 antikoerper
- Hintergrund
- This mAb recognizes a 60 kDa antigen associated with the mitochondria in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the mitochondria in human cells only. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.
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