CD79a Antikörper (AA 202-216)

Produktnummer ABIN302099

Der Maus Monoklonal Anti-CD79a-Antikörper wurde für FACS, IHC (p) und IHC (fro) validiert. Er ist geeignet, CD79a in Proben von Human, Maus, Ratte, Rind (Kuh), Schwein, Huhn, Kaninchen, Pferd, Meerschweinchen und Opossum zu detektieren. Es sind 5+ Publikationen verfügbar.

Kurzübersicht für CD79a Antikörper (AA 202-216) (ABIN302099)

Target: CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein, Huhn, Kaninchen, Pferd, Meerschweinchen, Opossum

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD79a Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: HM57

Produktdetails anti-CD79a Antikörper

Bindungsspezifität: AA 202-216

Verwendungszweck: Anti-Hu CD79a Purified

Spezifität: The antibody HM57 interacts with intracellular domain of CD79a (Ig alpha), a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms.

Kreuzreaktivität (Details): Human, Porcine, Mouse, Rat, Bovine, Equine (Horse), Guinea pig, Opossum, Rabbit, Chicken, Other not determined

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Synthetic peptide corresponding to amino acids 202-216 of human CD79a

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 5 μg/mL. Intracellular staining.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL.
Immunohistochemistry (frozen sections): Recommended dilution: 10 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-CD79a Antikörper (ABIN302099)

Faldyna, Samankova, Leva, Cerny, Oujezdska, Rehakova, Sinkora: "Cross-reactive anti-human monoclonal antibodies as a tool for B-cell identification in dogs and pigs." in: Veterinary immunology and immunopathology, Vol. 119, Issue 1-2, pp. 56-62, (2007) (PubMed).

Mason, Cordell, Brown, Borst, Jones, Pulford, Jaffe, Ralfkiaer, Dallenbach, Stein: "CD79a: a novel marker for B-cell neoplasms in routinely processed tissue samples." in: Blood, Vol. 86, Issue 4, pp. 1453-9, (1995) (PubMed).

Jones, Cordell, Beyers, Tse, Mason: "Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 12, pp. 5429-35, (1993) (PubMed).

Mason, van Noesel, Cordell, Comans-Bitter, Micklem, Tse, van Lier, van Dongen: "The B29 and mb-1 polypeptides are differentially expressed during human B cell differentiation." in: European journal of immunology, Vol. 22, Issue 10, pp. 2753-6, (1992) (PubMed).

Mason, Cordell, Tse, van Dongen, van Noesel, Micklem, Pulford, Valensi, Comans-Bitter, Borst: "The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 11, pp. 2474-82, (1991) (PubMed).

Details zu CD79a

Target: CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))

Andere Bezeichnung: CD79a

Hintergrund: CD79a molecule,CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.,BCR alpha, Ig-alpha, MB-1, IGA

Gen-ID: 973

UniProt: P11912

Pathways: BCR Signaling