GFP Antikörper

Produktnummer ABIN302066

Es sind 2+ Publikationen für dieses Produkt verfügbar. Der Kaninchen Polyklonal anti-GFP Antikörper wird verwendet zum Nachweis von GFP in Proben von Aequorea victoria. Er wurde validiert für WB, IP und ICC.

Kurzübersicht für GFP Antikörper (ABIN302066)

Target: GFP (Green Fluorescent Protein (GFP))

Reaktivität: Aequorea victoria

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GFP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-GFP Antikörper

Spezifität: The polyclonal antibody recognizes GFP, EGFP, EYFP fusion proteins in all species.

Kreuzreaktivität (Details): Recognizes fusion proteins in all species

Aufreinigung: Purified from rabbit serum by affinity chromatography

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: EGFP, a native full-length protein

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

Referenzen für anti-GFP Antikörper (ABIN302066)

Porrero, Rubio-Garrido, Avendauno, Clascua: "Mapping of fluorescent protein-expressing neurons and axon pathways in adult and developing Thy1-eYFP-H transgenic mice." in: Brain research, (2010) (PubMed).

Valenta, Lukas, Doubravska, Fafilek, Korinek: "HIC1 attenuates Wnt signaling by recruitment of TCF-4 and beta-catenin to the nuclear bodies." in: The EMBO journal, Vol. 25, Issue 11, pp. 2326-37, (2006) (PubMed).

Details zu GFP

Target: GFP (Green Fluorescent Protein (GFP))

Andere Bezeichnung: GFP

Hintergrund: Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (λ,=509 nm) when excited (excitation by Blue or UV light, absorption peak λ,=395 nm). GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. To increase a fluorescence intensity of GFP, chomophore mutations have been created. The EnhancedGFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. Yellow Fluorescent Protein, Cyan Fluorescent Protein) with warying spectral properties. Antibodies raised against full-length GFP variants should also detect other variants of the protein.