CD79a Antikörper (AA 202-216) (APC)

Produktnummer ABIN301989

Dieses Anti-CD79a-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD79a in ICFC. Geeignet für Human, Maus, Ratte, Rind (Kuh), Schwein, Huhn, Kaninchen, Pferd, Meerschweinchen und Opossum. Dieses Primary Antibody wurde in 6+ Publikationen zitiert.

Kurzübersicht für CD79a Antikörper (AA 202-216) (APC) (ABIN301989)

Target: CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein, Huhn, Kaninchen, Pferd, Meerschweinchen, Opossum

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD79a Antikörper ist konjugiert mit APC

Applikation: Intracellular Flow Cytometry (ICFC)

Klon: HM57

Produktdetails anti-CD79a Antikörper (APC)

Bindungsspezifität: AA 202-216

Verwendungszweck: Anti-Hu CD79a APC

Spezifität: The antibody HM57 interacts with intracellular domain of CD79a (Ig alpha), a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms.

Kreuzreaktivität (Details): Human, Porcine, Mouse, Rat, Bovine, Equine (Horse), Guinea pig, Opossum, Rabbit, Chicken, Other not determined

Aufreinigung: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Synthetic peptide corresponding to amino acids 202-216 of human CD79a

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.
Avoid prolonged exposure to light.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-CD79a Antikörper (APC) (ABIN301989)

Faldyna, Samankova, Leva, Cerny, Oujezdska, Rehakova, Sinkora: "Cross-reactive anti-human monoclonal antibodies as a tool for B-cell identification in dogs and pigs." in: Veterinary immunology and immunopathology, Vol. 119, Issue 1-2, pp. 56-62, (2007) (PubMed).

Mason, Cordell, Brown, Borst, Jones, Pulford, Jaffe, Ralfkiaer, Dallenbach, Stein: "CD79a: a novel marker for B-cell neoplasms in routinely processed tissue samples." in: Blood, Vol. 86, Issue 4, pp. 1453-9, (1995) (PubMed).

Jones, Cordell, Beyers, Tse, Mason: "Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 12, pp. 5429-35, (1993) (PubMed).

Mason, van Noesel, Cordell, Comans-Bitter, Micklem, Tse, van Lier, van Dongen: "The B29 and mb-1 polypeptides are differentially expressed during human B cell differentiation." in: European journal of immunology, Vol. 22, Issue 10, pp. 2753-6, (1992) (PubMed).

Mason, Cordell, Tse, van Dongen, van Noesel, Micklem, Pulford, Valensi, Comans-Bitter, Borst: "The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 11, pp. 2474-82, (1991) (PubMed).

van Noesel, van Lier, Cordell, Tse, van Schijndel, de Vries, Mason, Borst: "The membrane IgM-associated heterodimer on human B cells is a newly defined B cell antigen that contains the protein product of the mb-1 gene." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 11, pp. 3881-8, (1991) (PubMed).

Details zu CD79a

Target: CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))

Andere Bezeichnung: CD79a

Hintergrund: CD79a molecule,CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.,BCR alpha, Ig-alpha, MB-1, IGA

Gen-ID: 973

UniProt: P11912

Pathways: BCR Signaling