UBE2N Antikörper (AA 40-51)

Produktnummer ABIN298200

Der Ziege Polyklonal anti-UBE2N Antikörper (ABIN298200) detektiert spezifisch UBE2N in WB, ELISA, IHC, IF und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für UBE2N Antikörper (AA 40-51) (ABIN298200)

Target: UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser UBE2N Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-UBE2N Antikörper

Bindungsspezifität: AA 40-51

Spezifität: Amino acids 40-51 of human UBC13 protein.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from human UBE2N.

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:5000 - 1:25000), IF, IHC, IHC-P (2.5 μg/mL), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu UBE2N

Target: UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))

Andere Bezeichnung: UBE2N / UBC13

Hintergrund: Name/Gene ID: UBE2N

Synonyms: UBE2N, BLU, UbcH13, UbcH-ben, Ubiquitin carrier protein N, Yeast UBC13 homolog, UBC13, Ubiquitin-protein ligase N

Gen-ID: 7334

UniProt: P61088

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades, Positive Regulation of Response to DNA Damage Stimulus, Ubiquitin Proteasome Pathway