MAPK7 Antikörper (pThr218, Tyr220)

Produktnummer ABIN296169

Dieser Anti-MAPK7 Antikörper ist ein Schaf Polyklonal Antikörper zur Detektion von MAPK7 in WB. Geeignet für Human.

Kurzübersicht für MAPK7 Antikörper (pThr218, Tyr220) (ABIN296169)

Target: MAPK7 (Mitogen-Activated Protein Kinase 7 (MAPK7))

Reaktivität: Human

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser MAPK7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-MAPK7 Antikörper

Bindungsspezifität: AA 213-224, pThr218, Tyr220

Spezifität: Recognizes human phospho-BMK1/Erk5 (Thr218, Tyr220), Mr ~110kD. Predicted to cross-react with mouse and rat based on sequence homology.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to amino acids 213-224 of human BMK1/Erk5 (HQYFM[pT]E[pY]VATR), KLH-conjugated. The immunizing sequence is identical in mouse, 9/12aa identical in rat.

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: WB

Usage: Suitable for use in Western Blot. Western Blot: 1-:250-1:500 detects phospho-BMK1/Erk5 (Thr218, Tyr220) in RIPA lysates from HeLa cells treated with sorbitol. Lysates from untreated or sorbitol-treated HeLa cells were resolved by electrophoresis, transferred to nitrocellulose and probed with either anti-phospho-BMK1/Erk5 (Thr218, Tyr220) (1:500) or anti-BMK1/Erk5 (1:1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system (BMK1/Erk5 or phospho- BMK1/Erk5 (110kD).

Not recommended for: IHC-P

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 100 mM Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu MAPK7

Target: MAPK7 (Mitogen-Activated Protein Kinase 7 (MAPK7))

Andere Bezeichnung: MAPK7 / ERK5

Hintergrund: Name/Gene ID: MAPK7
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK7, Big MAP kinase 1, BMK-1, Erk4, ERK5, MAPK 7, PRKM7, BMK1, BMK1 kinase, ERK-5, MAP kinase 7

Gen-ID: 5598

UniProt: Q13164

Pathways: MAPK Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, cAMP Metabolic Process, Toll-Like Receptors Cascades, Negative Regulation of intrinsic apoptotic Signaling