GFP Antikörper

Produktnummer ABIN294468

Dieses Anti-GFP-Antikörper ist ein Huhn Polyklonal-Antikörper zur Detektion von GFP in WB, IP und ICC. Geeignet für Aequorea victoria.

Kurzübersicht für GFP Antikörper (ABIN294468)

Target: GFP (Green Fluorescent Protein (GFP))

Reaktivität: Aequorea victoria

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser GFP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-GFP Antikörper

Spezifität: Recognizes green fluorescent protein (GFP). Has been used to detect GFP in primary neurons and zebrafish fibroblasts.

Aufreinigung: Purified

Immunogen: Affinity purified, recombinant green fluorescent protein (GFP) containing a 6-His tag (single band, SDS-PAGE).

Isotyp: IgY

Anwendungsinformationen

Applikationshinweise: Approved: ICC, IP, WB

Usage: Suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation. Western Blot: Detects a band of the proper molecular weight, 30kD, in lysates from E. coli expressing GFP, but not in E. coli that do not express or in lysates prepared from E. coli containing the GFP-expressing plasmid but not induced, detect GFP in lysates from COS or primary neurons or fibroblast cells transfected with plasmids directing expression GFP or a tau-GFP fusion protein. Did not detect any signal when 50 μg of protein from a rat brain lysate (negative control) was fractionated on the SDS-PAGE. Titers were 1:5000-1:10000 when detected with an anti-chicken HRP conjugated secondary by ECL or by a colorimetric assay using DAB. Immunocytochemistry: GFP can be detected in cells that are transfected with a plasmid directing the expression of either GFP or a GFP-Fusion protein. Cells that do not express GFP exhibit no detectable staining. Binding was determined using HRP conjugated, anti-chicken secondary antibodies and visualized using DAB. The anti-GFP antibodies were used at dilutions of 1:1000-1:2500. Cells were fixed using 4 % formaldehyde in PBS,  pH 7.4. For immunocytochemistry, the cultures were fixed in 4 % formaldehyde in PBS,  pH 7.4, for 20 minutes at RT. The cells are permeabilized with 0.2 % Triton X-100 and 2 % serum (corresponding to the animal in which the secondary antibody was raised) in PBS for 20 minutes at RT. Cell cultures are then incubated with primary antisera in PBS containing 2 % serum (as above), at 4?C, overnight. Has also been used successfully with the avidin/biotin/peroxidase detection system. Cultures were washed four times (10 minutes each) between steps, and reaction product is developed with a 0.05 % diaminobenzidine/ 0.02 % hydrogen peroxide solution on ice for 8-10 minutes. Has been used to detect GFP in primary neurons and zebrafish fibroblasts.

Kommentare:

Target Species of Antibody: Aequorea victoria

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 10 mM sodium chloride, 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu GFP

Target: GFP (Green Fluorescent Protein (GFP))

Andere Bezeichnung: GFP

Hintergrund: Name/Gene ID: GFP

Synonyms: GFP

UniProt: P42212