GLI3 Antikörper
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- Target Alle GLI3 Antikörper anzeigen
- GLI3 (GLI Family Zinc Finger 3 (GLI3))
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Reaktivität
- Human, Maus
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Dieser GLI3 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
- Spezifität
- Recognizes human and mouse GLI-3 in the applications listed. In direct ELISAs and Western Blots, the antibody shows less than 1 % cross-reactivity with rhGLI-1 and rhGLI-2.
- Aufreinigung
- Affinity purified
- Immunogen
- E. coli-derived rhGLI-3.
- Isotyp
- IgG
- Top Product
- Discover our top product GLI3 Primärantikörper
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- Applikationshinweise
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Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in ELISA, Immunocytochemistry, and Western Blot. Western Blot: 0.1-0.2 μg/mL. Used in conjunction with the appropriate secondary reagents, detects human and mouse GLI-3. The detection limit for rhGLI-3 and rmGLI-3 is approximately 5 ng/lane under non- reducing and reducing conditions. Immunocytochemistry: 10 μg/mL detects human GLI-3 in HeLa cells. Cells were fixed with PBS containing 4 % paraformaldehyde and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA. After blocking, cells were incubated with diluted primary antibody followed by NorthernLights 557-coupled anti-goat IgG at room temperature in the dark. Between each step, cells were washed with PBS containing BSA. Direct ELISA: 0.5-1.0 μg/mL. Used in conjunction the appropriate secondary reagents, detects human and mouse GLI-3. The detection limit for rhGLI-3 and rmGLI-3 is approximately 1 ng/well. - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- deionized water
- Konzentration
- Lot specific
- Buffer
- Lyophilized.
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- Target
- GLI3 (GLI Family Zinc Finger 3 (GLI3))
- Andere Bezeichnung
- GLI3 (GLI3 Produkte)
- Synonyme
- ACLS antikoerper, GCPS antikoerper, GLI3-190 antikoerper, GLI3FL antikoerper, PAP-A antikoerper, PAPA antikoerper, PAPA1 antikoerper, PAPB antikoerper, PHS antikoerper, PPDIV antikoerper, xgli3 antikoerper, AI854843 antikoerper, AU023367 antikoerper, Bph antikoerper, Pdn antikoerper, Xt antikoerper, add antikoerper, im:7142603 antikoerper, GLI3 antikoerper, GLI family zinc finger 3 antikoerper, GLI family zinc finger 3 L homeolog antikoerper, GLI-Kruppel family member GLI3 antikoerper, GLI3 antikoerper, Gli3 antikoerper, gli3.L antikoerper, gli3 antikoerper
- Hintergrund
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Name/Gene ID: GLI3
Synonyms: GLI3, ACLS, GCPS, GLI-Kruppel family member GLI3, GLI3 full length protein, GLI3-190, GLI family zinc finger 3, Gli-3, GLI3FL, PAP-A, PAPA1, PHS, PPDIV, Transcriptional activator GLI3, PAPA, PAPB, GLI3 form of 190 kDa, Oncogene GLI3, Zinc finger protein GLI3 - Gen-ID
- 2737
- UniProt
- P10071
- Pathways
- Hedgehog Signalweg
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