Aurora A Antikörper (AA 394-403)

Produktnummer ABIN292641

Der Ziege Polyklonal anti-Aurora A Antikörper (ABIN292641) detektiert spezifisch Aurora A in WB, ELISA, IHC, IHC (p) und IP. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Aurora A Antikörper (AA 394-403) (ABIN292641)

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Aurora A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Produktdetails anti-Aurora A Antikörper

Bindungsspezifität: AA 394-403

Spezifität: Human AURKA / Aurora-A. Reported variants represent identical protein: NP_003591.2, NP_940835.1, NP_940836.1, NP_940837.1, NP_940838.1, NP_940839.1

Homologie: Percent identity by BLAST analysis: Human, Gorilla (100%) Gibbon, Marmoset (90%) Sheep, Bovine, Elephant, Panda, Helicobacter (80%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-QNKESASKQS from the C-terminus of human AURKA / Aurora-A (NP_003591.2). Percent identity by BLAST analysis: Human, Gorilla (100%), Gibbon, Marmoset (90%), Sheep, Bovine, Elephant, Panda, Helicobacter (80%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL), IP, Peptide-ELISA, WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Aurora A

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Andere Bezeichnung: AURKA / Aurora-A

Hintergrund: Name/Gene ID: AURKA
Subfamily: Aurora/IPL1-like
Family: Protein Kinase

Synonyms: AURKA, AIK, AIRK1, ARK1, Aurora-A kinase, Aurora kinase A, Aurora/IPL1-like kinase, AYK1, AURA, AURORA2, Breast tumor-amplified kinase, Breast-tumor-amplified kinase, BTAK, HARK1, IPL1-related kinase, Serine/threonine kinase 6, STK7, STK6, PPP1R47, ARK-1, Aurora 2, Aurora-A, Aurora-related kinase 1, Aurora/IPL1-related kinase 1, IAK1, Serine/threonine kinase 15, STK15

Gen-ID: 6790

NCBI Accession: NP_003591

UniProt: O14965

Pathways: Zellzyklus, Asymmetric Protein Localization