GFP Antikörper

Produktnummer ABIN285794

Der Kaninchen Polyklonal Anti-GFP-Antikörper wurde für WB und IP validiert. Er ist geeignet, GFP in Proben von Aequorea victoria zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für GFP Antikörper (ABIN285794)

Target: GFP (Green Fluorescent Protein (GFP))

Reaktivität: Aequorea victoria

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GFP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-GFP Antikörper

Spezifität: This antibody neutralizes and binds to human interferon alpha receptor with high affinity. It interacts with extracellular domain and blocks biological action of Type I interferons.

Kreuzreaktivität (Details): This antibody reacts with wild -type GFP and it's variants, such as EGFP, EBFP

Aufreinigung: purified

Immunogen: GFP antibody was raised in rabbit using E. Coli-expressed full length green fluorescent protein as the immunogen.

Anwendungsinformationen

Applikationshinweise: IP: 1:200-1:500, WB: 1:5,000
Optimal conditions should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitue in distilled water.

Konzentration: Lot specific

Buffer: Supplied as a lyophilized Potein A purified rabbit IgG with 0.1 % NaN3

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

Referenzen für anti-GFP Antikörper (ABIN285794)

Orozco, Tahirovic, Rentzsch, Schwenk, Haass, Edbauer: "Loss of fused in sarcoma (FUS) promotes pathological Tau splicing." in: EMBO reports, Vol. 13, Issue 8, pp. 759-64, (2012) (PubMed).

Details zu GFP

Target: GFP (Green Fluorescent Protein (GFP))

Andere Bezeichnung: GFP

Hintergrund: The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to blue light. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. In cell and molecular biology, the GFP gene is frequently used as a reporter of expression.