NHEJ1 Antikörper

Produktnummer ABIN2857060

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch NHEJ1 in WB, IF, IHC (p), IP und ICC. Er zeigt eine Reaktivität gegenüber Human und wurde in 1 Publikation erwähnt.

Kurzübersicht für NHEJ1 Antikörper (ABIN2857060)

Target: NHEJ1 (Nonhomologous End-Joining Factor 1 (NHEJ1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NHEJ1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-NHEJ1 Antikörper

Kreuzreaktivität: Human

Produktmerkmale: Rabbit polyclonal antibody to XLF (nonhomologous end-joining factor 1)
XLF antibody [N3C3]

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human XLF. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: HepG2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-NHEJ1 Antikörper (ABIN2857060)

Vu, Bonucci, Brenière, Cisneros-Aguirre, Pelupessy, Wang, Carlier, Bouvignies, Cortes, Aggarwal, Blackledge, Gueroui, Belle, Stark, Modesti, Ferrage: "Multivalent interactions of the disordered regions of XLF and XRCC4 foster robust cellular NHEJ and drive the formation of ligation-boosting condensates in vitro." in: Nature structural & molecular biology, Vol. 31, Issue 11, pp. 1732-1744, (2024) (PubMed).

Details zu NHEJ1

Target: NHEJ1 (Nonhomologous End-Joining Factor 1 (NHEJ1))

Andere Bezeichnung: non-homologous end joining factor 1

Hintergrund: Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders.

Cellular Localization: Nucleus

Molekulargewicht: 33 kDa

Gen-ID: 79840

UniProt: Q9H9Q4

Pathways: DNA Reparatur