UNC13B Antikörper (Internal Region)

Produktnummer ABIN2856793

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch UNC13B in WB, IF, IHC (p) und ICC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für UNC13B Antikörper (Internal Region) (ABIN2856793)

Target: UNC13B (Unc-13 Homolog B (UNC13B))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser UNC13B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Produktdetails anti-UNC13B Antikörper

Bindungsspezifität: Internal Region

Kreuzreaktivität: Maus, Ratte (Rattus), Rind (Kuh)

Kreuzreaktivität (Details): Mouse (99 %), Rat (98 %), Bovine (99 %)

Produktmerkmale: Rabbit Polyclonal antibody to UNC13B (unc-13 homolog B (C. elegans))
UNC13B antibody [N3C1], Internal

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human UNC13B. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Kommentare:

Positive Control: 293T , H1299 , HeLa , HepG2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu UNC13B

Target: UNC13B (Unc-13 Homolog B (UNC13B))

Andere Bezeichnung: UNC13B

Hintergrund: This gene is expressed in the kidney cortical epithelial cells and is upregulated by hyperglycemia. The encoded protein shares a high level of similarity to the rat homolog, and contains 3 C2 domains and a diacylglycerol-binding C1 domain. Hyperglycemia increases the levels of diacylglycerol, which has been shown to induce apoptosis in cells transfected with this gene and thus contribute to the renal cell complications of hyperglycemia. Studies in other species also indicate a role for this protein in the priming step of synaptic vesicle exocytosis.

Cellular Localization: Cytoplasm (By similarity) , Membrane, Peripheral membrane protein (By similarity) , Cell membrane (By similarity) , Cell junction , synapse (By similarity)

Molekulargewicht: 181 kDa

Gen-ID: 10497

Pathways: Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis