Nemo-Like Kinase Antikörper

Produktnummer ABIN2856544

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Nemo-Like Kinase in WB, IF, IHC (p) und ICC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Nemo-Like Kinase Antikörper (ABIN2856544)

Target: Nemo-Like Kinase (NLK)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Nemo-Like Kinase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Produktdetails anti-Nemo-Like Kinase Antikörper

Kreuzreaktivität: Human, Maus

Produktmerkmale: Rabbit Polyclonal antibody to NLK (nemo-like kinase)
NLK antibody [N3C3]

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human NLK. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: Mouse brain , HeLa , NLK-transfected 293T

Validation: Overexpression

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu Nemo-Like Kinase

Target: Nemo-Like Kinase (NLK)

Andere Bezeichnung: nemo like kinase

Hintergrund: Role in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP (By similarity). Involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3.

Cellular Localization: Nucleus (By similarity) , Cytoplasm (By similarity)

Molekulargewicht: 58 kDa

Gen-ID: 51701

UniProt: Q9UBE8

Pathways: Ubiquitin Proteasome Pathway