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PFKL Antikörper (Center)

Der Kaninchen Polyklonal Anti-PFKL-Antikörper wurde für WB, IF, IHC (p) und ICC validiert. Er ist geeignet, PFKL in Proben von Human und Maus zu detektieren.
Produktnummer ABIN2856164

Kurzübersicht für PFKL Antikörper (Center) (ABIN2856164)

Target

Alle PFKL Antikörper anzeigen
PFKL (Phosphofructokinase, Liver (PFKL))

Reaktivität

  • 44
  • 25
  • 21
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Maus

Wirt

  • 43
  • 6
Kaninchen

Klonalität

  • 44
  • 5
Polyklonal

Konjugat

  • 33
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser PFKL Antikörper ist unkonjugiert

Applikation

  • 37
  • 21
  • 19
  • 7
  • 6
  • 6
  • 4
  • 3
  • 1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
  • Bindungsspezifität

    • 8
    • 7
    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Center

    Kreuzreaktivität

    Rind (Kuh), Ratte (Rattus)

    Kreuzreaktivität (Details)

    Rat (95 %), Bovine (94 %)

    Produktmerkmale

    Rabbit Polyclonal antibody to PFKL (phosphofructokinase, liver)
    PFKL antibody [C1C3]

    Aufreinigung

    Purified by antigen-affinity chromatography.

    Immunogen

    Recombinant protein encompassing a sequence within the center region of human PFKL. The exact sequence is proprietary.

    Isotyp

    IgG
  • Applikationshinweise

    Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

    Kommentare

    Positive Control: A431 , H1299 , HeLa , HepG2

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    0.8 mg/mL

    Buffer

    0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

    Konservierungsmittel

    Thimerosal (Merthiolate)

    Vorsichtsmaßnahmen

    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Target

    PFKL (Phosphofructokinase, Liver (PFKL))

    Andere Bezeichnung

    PFKL

    Hintergrund

    Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. The protein encoded by this gene represents the L subunit. Alternate splicing results in two transcript variants, one of which is a candidate for nonsense-mediated decay (NMD).

    Molekulargewicht

    85 kDa

    Gen-ID

    5211

    Pathways

    Negative Regulation of Hormone Secretion, Warburg Effekt
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