PAK1 Antikörper (Center)

Produktnummer ABIN2855705

Für dieses Produkt ist 1 Publikation verfügbar. Der Kaninchen-Polyklonal Anti-PAK1-Antikörper wurde für WB, IF, IHC (p) und ICC validiert. Er ist geeignet zum Nachweis von PAK1 in Proben aus Human, Ratte und Maus. }

Kurzübersicht für PAK1 Antikörper (Center) (ABIN2855705)

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PAK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Produktdetails anti-PAK1 Antikörper

Bindungsspezifität: Center

Kreuzreaktivität: Rind (Kuh)

Kreuzreaktivität (Details): Bovine (100 %)

Produktmerkmale: Rabbit Polyclonal antibody to PAK1 (p21 protein (Cdc42/Rac)-activated kinase 1)
PAK1 antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human PAK1. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:10000*

Kommentare:

Positive Control: A431 , HeLa , BCL-1 , PC-12 , SH-SY5Y

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.48 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-PAK1 Antikörper (ABIN2855705)

Cai, Zhang, Zhang, Chen, Hu: "Effect of Th9/IL-9 on the growth of gastric cancer in nude mice." in: OncoTargets and therapy, Vol. 12, pp. 2225-2234, (2019) (PubMed).

Details zu PAK1

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Andere Bezeichnung: PAK1

Hintergrund: PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternativelt spliced transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Cytoplasm , Cell junction , focal adhesion

Molekulargewicht: 61 kDa

Gen-ID: 5058

Pathways: MAPK Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis