PRKAA2 Antikörper (Center)

Produktnummer ABIN2855639

Produktdetails anti-PRKAA2 Antikörper

Target: PRKAA2 (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2))

Bindungsspezifität: Center

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRKAA2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Kreuzreaktivität: Huhn, Schwein, Ratte (Rattus), Rind (Kuh), Xenopus tropicalis

Kreuzreaktivität (Details): Chicken (96 %), Pig (99 %), Rat (98 %), Bovine (99 %), Xenopus tropicalis (96 %)

Produktmerkmale: Rabbit Polyclonal antibody to AMPK alpha 2 (protein kinase, AMP-activated, alpha 2 catalytic subunit)
AMPK alpha 2 antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human AMPK alpha 2. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

Kommentare:

Positive Control: A431 , HeLa , A375 , NIH-3T3

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.55 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-PRKAA2 Antikörper (ABIN2855639)

Gao, Yuan, Wang, Lei, Shen, Cui, Zhang, Ding, Lu: "Metformin protects against PM2.5-induced lung injury and cardiac dysfunction independent of AMP-activated protein kinase α2." in: Redox biology, Vol. 28, pp. 101345, (2020) (PubMed).

Zhang, Zhang, Zhou, Fu, Michal, Ji, Du, Davis, Jiang: "Alternative polyadenylation drives genome-to-phenome information detours in the AMPKα1 and AMPKα2 knockout mice." in: Scientific reports, Vol. 8, Issue 1, pp. 6462, (2018) (PubMed).

Lin, Kiihl, Suhail, Liu, Chou, Kuang, Lu, Khor, Lin, Bader, Irizarry, Boeke: "Functional dissection of lysine deacetylases reveals that HDAC1 and p300 regulate AMPK." in: Nature, Vol. 482, Issue 7384, pp. 251-5, (2012) (PubMed).

Details zu PRKAA2

Target: PRKAA2 (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2))

Andere Bezeichnung: AMPK alpha 2 (PRKAA2 Produkte)

Synonyme: AMPK antikoerper, AMPK2 antikoerper, AMPKa2 antikoerper, PRKAA antikoerper, Ampk antikoerper, Ampka2 antikoerper, 2310008I11Rik antikoerper, A830082D05 antikoerper, AMPKalpha2 antikoerper, AMPKA2 antikoerper, ampk antikoerper, ampk2 antikoerper, prkaa antikoerper, prkaa1 antikoerper, PRKAA2 antikoerper, protein kinase AMP-activated catalytic subunit alpha 2 antikoerper, protein kinase, AMP-activated, alpha 2 catalytic subunit antikoerper, protein kinase, AMP-activated, alpha 2 catalytic subunit S homeolog antikoerper, PRKAA2 antikoerper, Prkaa2 antikoerper, prkaa2.S antikoerper, prkaa2 antikoerper

Hintergrund: The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Molekulargewicht: 62 kDa

Gen-ID: 5563

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, Warburg Effekt