GRP94 Antikörper (C-Term)

Produktnummer ABIN2855586

Dieses Anti-GRP94-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von GRP94 in WB, IF, IP, ICC und IHC (p). Geeignet für Human, Maus und Ratte. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für GRP94 Antikörper (C-Term) (ABIN2855586)

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GRP94 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-GRP94 Antikörper

Bindungsspezifität: C-Term

Kreuzreaktivität: Hund, Affe, Schwein

Kreuzreaktivität (Details): Dog (95 %), Monkey (98 %), Pig (95 %)

Produktmerkmale: Rabbit Polyclonal antibody to GRP94 (heat shock protein 90 kDa beta (Grp94), member 1)
GRP94 antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human GRP94. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

Kommentare:

Positive Control: 293T , A431 , HeLa , HepG2 , NIH-3T3 , Mouse brain , PC-12 , Rat-2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-GRP94 Antikörper (ABIN2855586)

Brzozowa-Zasada, Kurek, Piecuch, Wyrobiec: "The clinical and prognostic evaluation of GRP94 immunoexpression in Caucasian patients with colorectal adenocarcinoma." in: Przeglad gastroenterologiczny, Vol. 14, Issue 2, pp. 140-147, (2019) (PubMed).

Details zu GRP94

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Andere Bezeichnung: GRP94

Hintergrund: HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 is an endoplasmic reticulum HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1, MIM 140571) and mitochondria (TRAP1, MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM]

Cellular Localization: Endoplasmic reticulum lumen , Melanosome

Molekulargewicht: 92 kDa

Gen-ID: 7184

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades