CDK4 Antikörper

Produktnummer ABIN2855529

Dieses Anti-CDK4-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CDK4 in WB, IF, ICC und IHC (p). Geeignet für Human. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für CDK4 Antikörper (ABIN2855529)

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDK4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Güteklasse: KO Validated

Produktdetails anti-CDK4 Antikörper

Kreuzreaktivität: Rind (Kuh), Human, Maus, Ratte

Produktmerkmale: Rabbit Polyclonal antibody to CDK4 (cyclin-dependent kinase 4)
CDK4 antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human CDK4. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: PC-12 , 293T

Validation: KO/KD, Orthogonal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.82 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-CDK4 Antikörper (ABIN2855529)

Chen, Huang, Lin, Fang: "Piperlongumine induces autophagy in biliary cancer cells via reactive oxygen species-activated Erk signaling pathway." in: International journal of molecular medicine, Vol. 44, Issue 5, pp. 1687-1696, (2020) (PubMed).

Li, Du, Aldana-Masangkay, Wang, Urak, Forman, Rosen, Chen: "Regulation of miR-34b/c-targeted gene expression program by SUMOylation." in: Nucleic acids research, Vol. 46, Issue 14, pp. 7108-7123, (2019) (PubMed).

Details zu CDK4

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Andere Bezeichnung: cyclin dependent kinase 4

Hintergrund: The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.

Molekulargewicht: 34 kDa

Gen-ID: 1019

UniProt: P11802

Pathways: Zellzyklus, Mitotic G1-G1/S Phases, Regulation of Cell Size