BTRC Antikörper

Produktnummer ABIN2855453

Der Kaninchen Polyklonal Anti-BTRC-Antikörper wurde für WB und IHC (p) validiert. Er ist geeignet, BTRC in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für BTRC Antikörper (ABIN2855453)

Target: BTRC (beta-Transducin Repeat Containing (BTRC))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BTRC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-BTRC Antikörper

Kreuzreaktivität: Human, Ratte

Produktmerkmale: Rabbit polyclonal antibody to beta-TrCP (beta-transducin repeat containing)
beta-TrCP antibody [N1C1]

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human beta-TrCP. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Validation: Orthogonal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-BTRC Antikörper (ABIN2855453)

Lin, Chang, Lee, Campbell, Wang, Shen, Chung, Chang, Chiu, Pan, Lin, Chu, Kung, Cheng, Chang: "REST reduction is essential for hypoxia-induced neuroendocrine differentiation of prostate cancer cells by activating autophagy signaling." in: Oncotarget, Vol. 7, Issue 18, pp. 26137-51, (2018) (PubMed).

Details zu BTRC

Target: BTRC (beta-Transducin Repeat Containing (BTRC))

Andere Bezeichnung: beta-transducin repeat containing E3 ubiquitin protein ligase

Hintergrund: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class, in addition to an F-box, this protein contains multiple WD-40 repeats. This protein is homologous to Xenopus bTrCP1, yeast Met30, Neurospora Scon2 and Drosophila Slimb proteins. It interacts with HIV-1 Vpu and connects CD4 to the proteolytic machinery. It also associates specifically with phosphorylated IkappaBalpha and beta-catenin destruction motifs, probably functioning in multiple transcriptional programs by activating the NF-kappaB pathway and inhibiting the beta-catenin pathway.

Cellular Localization: Cytoplasm

Molekulargewicht: 69 kDa

Gen-ID: 8945

UniProt: Q9Y297

Pathways: Zellzyklus, Hedgehog Signalweg