POLR2B Antikörper (N-Term)

Produktnummer ABIN2855420

Der Kaninchen Polyklonal Anti-POLR2B-Antikörper wurde für WB, ICC, IF und IHC (p) validiert. Er ist geeignet, POLR2B in Proben von Human und Maus zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für POLR2B Antikörper (N-Term) (ABIN2855420)

Target: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POLR2B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-POLR2B Antikörper

Bindungsspezifität: N-Term

Kreuzreaktivität: Ratte (Rattus), Rhesusaffen, Rind (Kuh)

Kreuzreaktivität (Details): Rat (100 %), Rhesus Monkey (100 %), Bovine (100 %)

Produktmerkmale: Rabbit Polyclonal antibody to RNA polymerase IIB (DNA-directed RNA polymerase II subunit RPB2)
RPB2 antibody [N1], N-term

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human RPB2. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Kommentare:

Positive Control: H1299 , BCL-1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.65 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-POLR2B Antikörper (ABIN2855420)

Jönsson, Ludvik Brattås, Gustafsson, Petri, Yudovich, Pircs, Verschuere, Madsen, Hansson, Larsson, Månsson, Meissner, Jakobsson: "Activation of neuronal genes via LINE-1 elements upon global DNA demethylation in human neural progenitors." in: Nature communications, Vol. 10, Issue 1, pp. 3182, (2019) (PubMed).

Details zu POLR2B

Target: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Andere Bezeichnung: RPB2

Hintergrund: This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.

Cellular Localization: Nucleus

Molekulargewicht: 134 kDa

Gen-ID: 5431

Pathways: Regulatorische RNA Pathways, DNA Reparatur