ARPC2 Antikörper

Produktnummer ABIN2855249

Der Kaninchen Polyklonal Anti-ARPC2-Antikörper wurde für WB, IF, ICC und IHC (p) validiert und wurde unabhängig validiert. Er ist geeignet, ARPC2 in Proben von Human zu detektieren.

Kurzübersicht für ARPC2 Antikörper (ABIN2855249)

Target: ARPC2 (Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ARPC2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ARPC2 Antikörper

Kreuzreaktivität: Human, Maus, Ratte, Zebrafisch (Danio rerio)

Produktmerkmale: Rabbit Polyclonal antibody to ARPC2 (actin related protein 2/3 complex, subunit 2, 34 kDa)
ARPC2 antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ARPC2. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:5000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: rat kidney , mouse kidney

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (mIHC)

Validierung #104342 (Multiplex Immunohistochemistry)

by: Akoya Biosciences

No.: #104342

Datum: 20.04.2021

Antigen: ARPC2

Chargennummer: 39911

Validierte Anwendung: Multiplex Immunohistochemistry

Positivkontrolle:

FFPE normal human cortex

Negativkontrolle:

Unlabeled control (FFPE normal human cortex)

Bewertung:

Passed. The anti-ARPC2 antibody ABIN2855249 produced staining in neuronal processes throughout the human cortex.

Validierungsbilder

FFPE normal human cortex tissue section labeled with anti-ARPC2 antibody ABIN2855249 (cyan; conjugated to fluorophore AF488) after EDTA antigen retrieval. MAP2, neurofilament, and GFAP were labeled with anti-MAP2 antibody ABIN125739 (green; conjugated to fluorophore ATTO 550), anti-neurofilament antibody (red; conjugated to fluorophore AF488), and anti-GFAP antibody (purple; conjugated to fluorophore AF647) respectively.

FFPE normal human cortex tissue section labeled with anti-ARPC2 antibody ABIN2855249 (cyan; conjugated to fluorophore AF488) after EDTA antigen retrieval. GFAP was labeled with anti-ARPC2 antibody (red; conjugated to fluorophore AF647).

Protokoll

Primärantikörper: ABIN2855249

Full Protocol:

• Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
• Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual fresh-frozen tissue protocol.
• Conjugation of the anti-ARPC2 antibody ABIN2855249 to an oligo barcode used to bind oligo-conjugated fluorophore AF488.

Anmerkungen:

• No signal was detected in unlabeled specimens.

• Specific staining of ARPC2 was observed with human FFPE cortical tissue sections with tris-EDTA antigen retrieval.

• Anti-ARPC antibody ABIN2855249 was weakly compatible with staining of fresh frozen mouse tissue

• Optimal staining and signal to noise ratios were obtained if tissue was pre-treated for autofluorescence removal (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu ARPC2

Target: ARPC2 (Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2))

Andere Bezeichnung: actin related protein 2/3 complex subunit 2

Hintergrund: This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined.

Cellular Localization: Cytoplasm , cytoskeleton , Cell projection

Molekulargewicht: 34 kDa

Gen-ID: 10109

UniProt: O15144

Pathways: RTK Signalweg, Regulation of Actin Filament Polymerization