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EIF2A Antikörper (Center)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch EIF2A in WB, IF und ICC. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 1 Publikation erwähnt.
Produktnummer ABIN2854984

Kurzübersicht für EIF2A Antikörper (Center) (ABIN2854984)

Target

Alle EIF2A Antikörper anzeigen
EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Reaktivität

  • 83
  • 41
  • 41
  • 8
  • 5
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Maus, Ratte

Wirt

  • 67
  • 15
  • 2
Kaninchen

Klonalität

  • 64
  • 20
Polyklonal

Konjugat

  • 51
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser EIF2A Antikörper ist unkonjugiert

Applikation

  • 66
  • 38
  • 33
  • 29
  • 18
  • 13
  • 13
  • 11
  • 6
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
  • Bindungsspezifität

    • 17
    • 8
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Center

    Kreuzreaktivität

    Rhesusaffen

    Kreuzreaktivität (Details)

    Rhesus Monkey (100 %)

    Produktmerkmale

    Rabbit Polyclonal antibody to eIF2 alpha (eukaryotic translation initiation factor 2, subunit 1 alpha, 35 kDa)
    eIF2 alpha antibody [N1C3]

    Aufreinigung

    Purified by antigen-affinity chromatography.

    Immunogen

    Recombinant protein encompassing a sequence within the center region of human eIF2 alpha. The exact sequence is proprietary.

    Isotyp

    IgG
  • Applikationshinweise

    Suggested dilution Reference ICC/IF 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Western blot1:500-1:3000*

    Kommentare

    Positive Control: H1299 , Raji , *rat neuron , *HeLa , *N2a ,

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    0.31 mg/mL

    Buffer

    0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

    Konservierungsmittel

    Thimerosal (Merthiolate)

    Vorsichtsmaßnahmen

    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Wu, Yen, Kou, Wu: "Luteolin and Apigenin Attenuate 4-Hydroxy-2-Nonenal-Mediated Cell Death through Modulation of UPR, Nrf2-ARE and MAPK Pathways in PC12 Cells." in: PLoS ONE, Vol. 10, Issue 6, pp. e0130599, (2016) (PubMed).

  • Target

    EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

    Andere Bezeichnung

    eIF2 alpha

    Hintergrund

    The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM]

    Molekulargewicht

    36 kDa

    Gen-ID

    1965

    Pathways

    Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly
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