BRCA1 Antikörper (C-Term)

Produktnummer ABIN2854845

Dieses Anti-BRCA1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von BRCA1 in ELISA, IF und ICC. Geeignet für Human.

Kurzübersicht für BRCA1 Antikörper (C-Term) (ABIN2854845)

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BRCA1 Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-BRCA1 Antikörper

Bindungsspezifität: C-Term

Kreuzreaktivität: Hund, Rhesusaffen, Schimpanse

Kreuzreaktivität (Details): Dog (87 %), Rhesus Monkey (100 %), Chimpanzee (100 %)

Produktmerkmale: Rabbit Polyclonal antibody to BRCA1 (breast cancer 1, early onset)
BRCA1 antibody [C3], C-term

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human BRCA1. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference ICC/IF 1:100-1:1000* ELISA-Ag Assay-dependent dilution Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* ELISA-AgAssay-dependent dilution

Kommentare:

Positive Control: Synthetic peptide: 1:8,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu BRCA1

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Andere Bezeichnung: BRCA1

Hintergrund: This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC for BRCA1-associated genome surveillance complex. This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complex. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants have been described for this gene but only some have had their full-length natures identified.

Cellular Localization: Nucleus , Isoform 3: Cytoplasm , Isoform 5: Cytoplasm

Molekulargewicht: 208 kDa

Gen-ID: 672

Pathways: Zellzyklus, DNA Reparatur, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus