DKK3 Antikörper (N-Term)

Produktnummer ABIN2798864

Der Kaninchen Polyklonal Anti-DKK3-Antikörper wurde für WB und IHC validiert. Er ist geeignet, DKK3 in Proben von Human zu detektieren.

Kurzübersicht für DKK3 Antikörper (N-Term) (ABIN2798864)

Target: DKK3

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DKK3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-DKK3 Antikörper

Bindungsspezifität: AA 15-45, N-Term

Verwendungszweck: Rabbit Anti-Human DKK3 (N-term) Antibody

Immunogen: This DKK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-45 amino acids from the N-terminal region of human DKK3.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Western Blot, Immunohistochemistry
Recommended Dilutions
WB: 1:1000, IHC: 1:50-100Western blot analysis of lysates from 293, 293T, A549, HL-60, MCF-7 cell line and mouse brain tissue lysate (from left to right), using DKK3 Antibody (A30), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.500 mg/mL

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C (short-term), -20°C (long-term)

Details zu DKK3

Target: DKK3

Hintergrund:

Target Description: DKK3, like DKK1, DKK2, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed wide expression of the DKK3 transcript, with highest expression in heart, brain, and spinal cord. In situ hybridization reveals highest expression in mouse brain, eye, and heart.

Gene Symbol: DKK3

Molekulargewicht: 38390 Da

Gen-ID: 27122

UniProt: Q9UBP4