CDK9 Antikörper (N-Term)

Produktnummer ABIN2792147

Produktdetails anti-CDK9 Antikörper

Target: CDK9 (Cyclin-Dependent Kinase 9 (CDK9))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein, Hund, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDK9 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Sequenz: MAKQYDSVEC PFCDEVSKYE KLAKIGQGTF GEVFKARHRK TGQKVALKKV

Homologie: Cow: 92%, Dog: 100%, Human: 100%, Mouse: 92%, Pig: 92%, Rat: 92%, Zebrafish: 92%

Produktmerkmale: This is a rabbit polyclonal antibody against CDK9. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CDK9

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 372 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu CDK9

Target: CDK9 (Cyclin-Dependent Kinase 9 (CDK9))

Andere Bezeichnung: CDK9 (CDK9 Produkte)

Hintergrund: CDK9 is the member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: C-2k, CDC2L4, PITALRE, TAK, CTK1
Protein Interaction Partner: UBC, MEPCE, tat, LARP7, BRD4, CCNT1, ATM, SUMO2, AFF1, HEXIM1, FKBP5, RNF20, MSL2, RNF40, HIST2H2BE, POLR2A, MSL1, CDK9, SMC2, PLEC, CCAR2, SMC4, STIP1, MLLT3, MLLT1, CDK12, AFF4, FGFR1, CCNT2, AFF3, FBXO25, CUL1, SKIV2L, ATP1A1, SUPT5H, RPS4X, RN7SK, HEX
Protein Size: 372

Molekulargewicht: 43 kDa

Gen-ID: 1025

NCBI Accession: NM_001261, NP_001252

UniProt: P50750

Pathways: Zellzyklus