IDH1 Antikörper (C-Term)

Produktnummer ABIN2785883

Der Kaninchen Polyklonal Anti-IDH1-Antikörper wurde für WB und IHC validiert. Er ist geeignet, IDH1 in Proben von Human, Maus, Ratte, Hund, Pferd, Meerschweinchen, Rind (Kuh), Kaninchen und Schaf zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für IDH1 Antikörper (C-Term) (ABIN2785883)

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Reaktivität: Human, Maus, Ratte, Hund, Pferd, Meerschweinchen, Rind (Kuh), Kaninchen, Schaf

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IDH1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-IDH1 Antikörper

Bindungsspezifität: C-Term

Sequenz: VSIETIEAGF MTKDLAACIK GLPNVQRSDY LNTFEFMDKL GENLKIKLAQ

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against IDH1. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human IDH1

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 414 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-IDH1 Antikörper (ABIN2785883)

Jena, Janjanam, Naru, Kumar, Kumar, Singh, Mohapatra, Kola, Anand, Jaswal, Verma, Malakar, Dang, Kaushik, Reddy, Mohanty: "DIGE based proteome analysis of mammary gland tissue in water buffalo (Bubalus bubalis): Lactating vis-a-vis heifer." in: Journal of proteomics, Vol. 119C, pp. 100-111, (2015) (PubMed).

Details zu IDH1

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Andere Bezeichnung: IDH1

Hintergrund: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+).The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: IDH, IDP, PICD, IDCD, IDPC
Protein Interaction Partner: SUMO2, UBC, GLRX3, UBE2H, THOP1, TALDO1, RCN1, RBBP7, RANBP1, PTMS, OXCT1, NME1, HSPE1, GOT1, FDPS, DCK, ANXA6, ADSS, NME1-NME2, EFHD2, C12orf10, SBDS, CHMP4A, CBX3, SUGT1, PEX5, AK2, AK1, MDH2, ASF1B, CDK2, SLC2A4, ELAVL1, SUMO4, ZHX1,
Protein Size: 414

Molekulargewicht: 47 kDa

Gen-ID: 3417

NCBI Accession: NM_005896, NP_005887

UniProt: O75874

Pathways: Warburg Effekt