HYAL1 Antikörper (N-Term)

Produktnummer ABIN2784343

Dieses unabhängig validierte Kaninchen Polyklonal-Antikörper erkennt spezifisch HYAL1 in WB und IHC. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Rind (Kuh), Hund, Meerschweinchen, Pferd und Kaninchen.

Kurzübersicht für HYAL1 Antikörper (N-Term) (ABIN2784343)

Target: HYAL1 (Hyaluronidase-1 (HYAL1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Meerschweinchen, Pferd, Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HYAL1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-HYAL1 Antikörper

Bindungsspezifität: N-Term

Sequenz: TIFYSSQLGT YPYYTPTGEP VFGGLPQNAS LIAHLARTFQ DILAAIPAPD

Homologie: Cow: 86%, Dog: 85%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 92%, Rabbit: 93%, Rat: 92%

Produktmerkmale: This is a rabbit polyclonal antibody against HYAL1. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HYAL1

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 435 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (IHC)

Validierung #100034 (Immunohistochemistry)

by: University of Manitoba, Max Rady College of Medicine Biochemistry and Medical Genetics, Department of Biochemistry and Medical Genetics

No.: #100034

Datum: 16.08.2016

Antigen: HYAL1 antibody - N-terminal region

Chargennummer: QC23783

Validierte Anwendung: Immunohistochemistry

Positivkontrolle: Testes of Hyal1 +/+ mouse

Negativkontrolle: Testes of Hyal1 -/- mouse

Bewertung: ABIN2784343 does detect mouse HYAL1 by immunohistochemistry in paraffin sections. The immunohistochemistry is based on increased signal in wild type mouse testes compared to Hyal1-/- mouse testes.

Validierungsbilder

Testes of Hyal1 -/- mouse (A) or Hyal1 +/+ mouse (B) stained with ABIN2784342 as described in the protocol.

Protokoll

Primärantikörper: ABIN2784343

Sekundärantikörper: Goat-anti-rabbit AF568

Full Protocol:

• Deparafinize and rehydrate:
• xylene (I & II) – 2x 5min
• 100% Ethanol (I & II) – 2x 5min
• 95% Ethanol (I & II) – 2x 3min
• 70% Ethanol (I) – 3min
• 50% Ethanol (I) – 3min
• ddH₂O – 3min
• Antigen retrieval:
• Immerse slides in citrate buffer (10mM Sodium citrate at pH6.0) preheated to 95°C.
• Incubate at 95°C for 20min.
• Cool down at RT for 20min.
• Wash twice with PBS for 5min.
• Immunostaining:
• Block in blocking solution (prepare 3% BSA in TBST) at RT for 60min.
• Incubate sections with primary Hyaluronidase-1 (HYAL1) (N-Term) antibody ABIN2784343 diluted 1:200 in blocking solution.
• Incubate at 4°C overnight.
• Wash slides three times with TBST 5min.
• Incubate sections with goat-anti-rabbit AF568 secondary antibody conjugate diluted 1:500 in blocking solution.
• Incubate at RT for 1h.
• Wash slides in TBST - 1x 5min at RT.
• Wash three times with H₂O for 5min.
• Incubated in Hoechst solution diluted 1:100.000 for 8-9min.
• Wash twice with H₂O for 5min.
• Mounted with ProLong Gold (ThermoFisher, P36931) solution.

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu HYAL1

Target: HYAL1 (Hyaluronidase-1 (HYAL1))

Andere Bezeichnung: HYAL1

Hintergrund: HYAL1 is a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. This gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene.This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: HYAL-1, LUCA1, MGC45987, NAT6
Protein Interaction Partner: UBC, COL2A1,
Protein Size: 435

Molekulargewicht: 45 kDa

Gen-ID: 3373

NCBI Accession: NM_153282, NP_695014

UniProt: Q12794

Pathways: Glycosaminoglycan Metabolic Process