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Antigen | HYAL1 antibody - N-terminal region |
Chargennummer | QC25700 |
Validierte Anwendung | Western Blotting |
Positivkontrolle | Hyal1 +/+ mouse liver lysates, Hyal1 -/- mouse embryonic fibroblasts liver lysates overexpressing human HYAL1 |
Negativkontrolle | Hyal1 -/- mouse liver lysates, Hyal1 -/- mouse embryonic fibroblasts |
Bewertung | ABIN2784342 detects endogenous mouse HYAL1, and overexpressed human HYAL1 by Western blot analysis. The positive western blot assessment is based on the detection of a band of the appropriate molecular mass in Hyal1+/+ but not Hyal1-/- mouse tissue and the presence of a band of the appropriate molecular mass in Hyal1-/- cells transfected with human HYAL1. The identify of these bands was confirmed using the 1D10 antibody that had previously been shown to detect HYAL1. There are background bands to be considered in the interpretation of the blot. |
Primärantikörper | ABIN2784342 |
Sekundärantikörper | Peroxidase AffiniPure Donkey Anti-Rabbit IgG (H+L) (Jackson Labs, 711-035-152, Lot 106483) |
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