PDHA1 Antikörper (C-Term)

Produktnummer ABIN2783236

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch PDHA1 in WB. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Rind (Kuh), Zebrafisch (Danio rerio), Schwein, Hund, Meerschweinchen, Kaninchen und Pferd.

Kurzübersicht für PDHA1 Antikörper (C-Term) (ABIN2783236)

Target: PDHA1 (Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Zebrafisch (Danio rerio), Schwein, Hund, Meerschweinchen, Kaninchen, Pferd

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PDHA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-PDHA1 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: PDHA1 Antibody - C-terminal region

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%, Zebrafish: 82%

Produktmerkmale: This is a rabbit polyclonal antibody against PDHA1. It was validated on Western Blot using a cell lysate as a positive control.

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human PDHA1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8109733

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Details zu PDHA1

Target: PDHA1 (Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1))

Andere Bezeichnung: PDHA1

Hintergrund:

Targeted Subunit: alpha, somatic form, mitochondrial

Background Information: The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1), dihydrolipoyl transacetylase (DLAT), and dihydrolipoyl dehydrogenase (DLD). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex.The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1), dihydrolipoyl transacetylase (DLAT, MIM 608770) (E2, EC 2.3.1.12), and dihydrolipoyl dehydrogenase (DLD, MIM 238331) (E3, EC 1.8.1.4). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Pyruvate dehydrogenase (lipoamide) alpha 1

Alternative Symbols: PDHA, PDHAD, PHE1A, PDHCE1A

Protein Name: Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial

Molekulargewicht: 40kDa

Gen-ID: 5160

NCBI Accession: NP_000275

UniProt: P08559

Pathways: Warburg Effekt