MTHFD1 Antikörper (N-Term)

Produktnummer ABIN2782514

Produktdetails anti-MTHFD1 Antikörper

Target: MTHFD1 (Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 1, Methenyltetrahydrofolate Cyclohydrolase, Formyltetrahydrofolate Synthetase (MTHFD1))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte, Kaninchen, Pferd, Zebrafisch (Danio rerio), Hund, Meerschweinchen, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MTHFD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Sequenz: RTTTESEVMK YITSLNEDST VHGFLVQLPL DSENSINTEE VINAIAPEKD

Homologie: Cow: 92%, Dog: 92%, Guinea Pig: 92%, Horse: 92%, Human: 100%, Mouse: 100%, Rabbit: 85%, Rat: 100%, Zebrafish: 85%

Produktmerkmale: This is a rabbit polyclonal antibody against MTHFD1. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MTHFD1

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 935 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-MTHFD1 Antikörper (ABIN2782514)

Liu, Shah, Fan, Park, Wellen, Rabinowitz: "Malic enzyme tracers reveal hypoxia-induced switch in adipocyte NADPH pathway usage." in: Nature chemical biology, Vol. 12, Issue 5, pp. 345-52, (2016) (PubMed).

Details zu MTHFD1

Target: MTHFD1 (Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 1, Methenyltetrahydrofolate Cyclohydrolase, Formyltetrahydrofolate Synthetase (MTHFD1))

Andere Bezeichnung: MTHFD1 (MTHFD1 Produkte)

Hintergrund: MTHFD1 possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain.This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: MTHFC, MTHFD
Protein Interaction Partner: UBC, FUS, SUMO2, SUMO3, NEDD8, MDM2, RPA3, RPA2, RPA1, RNF2, RPS21, MAT2A, UBQLN1, FBXO6, UBD, ADRB2, CD81, VCAM1, HSP90AA1, HSP90AB1, PRKCDBP, SFXN1, TMEM165, IARS2, SARS2, SF3B3, FAF2, PPT2, SNRNP70, RPL32, MRPL23, ALDH18A1, PRPH, PC, ICT1, GAA, ACSL3,
Protein Size: 935

Molekulargewicht: 101 kDa

Gen-ID: 4522

NCBI Accession: NM_005956, NP_005947

UniProt: P11586

Pathways: Methionine Biosynthetic Process