Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

Sonic Hedgehog Antikörper (N-Term)

Der Kaninchen Polyklonal Anti-Sonic Hedgehog-Antikörper wurde für WB und IHC validiert. Er ist geeignet, Sonic Hedgehog in Proben von Human, Maus, Ratte, Zebrafisch (Danio rerio), Hund, Rind (Kuh), Meerschweinchen, Pferd, Ziege und Kaninchen zu detektieren.
Produktnummer ABIN2781775

Kurzübersicht für Sonic Hedgehog Antikörper (N-Term) (ABIN2781775)

Target

Alle Sonic Hedgehog (SHH) Antikörper anzeigen
Sonic Hedgehog (SHH)

Reaktivität

  • 80
  • 56
  • 42
  • 17
  • 9
  • 5
  • 5
  • 5
  • 4
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Maus, Ratte, Zebrafisch (Danio rerio), Hund, Rind (Kuh), Meerschweinchen, Pferd, Ziege, Kaninchen

Wirt

  • 84
  • 22
  • 9
  • 1
  • 1
Kaninchen

Klonalität

  • 76
  • 41
Polyklonal

Konjugat

  • 72
  • 10
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser Sonic Hedgehog Antikörper ist unkonjugiert

Applikation

  • 89
  • 44
  • 31
  • 19
  • 19
  • 14
  • 13
  • 12
  • 11
  • 10
  • 5
  • 4
  • 3
  • 1
Western Blotting (WB), Immunohistochemistry (IHC)
  • Bindungsspezifität

    • 15
    • 11
    • 9
    • 8
    • 6
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term

    Sequenz

    RCLLLVLVSS LLVCSGLACG PGRGFGKRRH PKKLTPLAYK QFIPNVAEKT

    Homologie

    Cow: 93%, Dog: 100%, Goat: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

    Produktmerkmale

    This is a rabbit polyclonal antibody against SHH. It was validated on Western Blot and immunohistochemistry.

    Aufreinigung

    Affinity Purified

    Immunogen

    The immunogen is a synthetic peptide directed towards the N terminal region of human SHH
  • Applikationshinweise

    Optimal working dilutions should be determined experimentally by the investigator.

    Kommentare

    Antigen size: 462 AA

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    Lot specific

    Buffer

    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freeze-thaw cycles.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Target

    Sonic Hedgehog (SHH)

    Andere Bezeichnung

    SHH

    Hintergrund

    SHH is a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE). It is also thought that mutations in its gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.This gene, which is expressed only during embryogenesis, encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. In addition, it is thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.
    Alias Symbols: HHG1, HLP3, HPE3, SMMCI, TPT, TPTPS, MCOPCB5
    Protein Interaction Partner: UBC, SEL1L, DERL2, DERL1, SYVN1, HHIP, PTCH2, PTCH1, SHH, ADCYAP1, GAS1,
    Protein Size: 462

    Molekulargewicht

    28 kDa

    Gen-ID

    6469

    NCBI Accession

    NM_000193, NP_000184

    UniProt

    Q15465

    Pathways

    Hedgehog Signalweg, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
Sie sind hier:
Chat with us!