Cyclin D1 Antikörper (Middle Region)

Produktnummer ABIN2779291

Der Kaninchen Polyklonal anti-Cyclin D1 Antikörper wird verwendet zum Nachweis von Cyclin D1 in Proben von Human, Ratte, Rind (Kuh), Hund, Zebrafisch (Danio rerio), Pferd, Kaninchen, Schwein und Saccharomyces cerevisiae. Er wurde validiert für WB.

Kurzübersicht für Cyclin D1 Antikörper (Middle Region) (ABIN2779291)

Target: Cyclin D1 (CCND1)

Reaktivität: Human, Ratte, Rind (Kuh), Hund, Zebrafisch (Danio rerio), Pferd, Kaninchen, Schwein, Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cyclin D1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Cyclin D1 Antikörper

Bindungsspezifität: Middle Region

Verwendungszweck: CCND1 Antibody - middle region

Sequenz: ACQEQIEALL ESSLRQAQQN MDPKAAEEEE EEEEEVDLAC TPTDVRDVDI

Homologie: Cow: 92%, Dog: 92%, Horse: 92%, Human: 100%, Pig: 91%, Rabbit: 83%, Rat: 85%, Yeast: 83%, Zebrafish: 79%

Produktmerkmale: This is a rabbit polyclonal antibody against CCND1. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CCND1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP30163-100UG

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Details zu Cyclin D1

Target: Cyclin D1 (CCND1)

Andere Bezeichnung: CCND1

Hintergrund:

Background Information: CCND1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Cyclin D1

Alternative Symbols: BCL1, PRAD1, U21B31, D11S287E

Protein Name: G1/S-specific cyclin-D1

Molekulargewicht: 34kDa

Gen-ID: 595

NCBI Accession: NP_444284

UniProt: P24385

Pathways: PI3K-Akt Signalweg, Zellzyklus, Mitotic G1-G1/S Phases, ER-Nucleus Signaling