Calreticulin Antikörper (C-Term)

Produktnummer ABIN2779284

Der Kaninchen Polyklonal anti-Calreticulin Antikörper (ABIN2779284) detektiert spezifisch Calreticulin in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte, Maus, Hund, Meerschweinchen, Rind (Kuh), Pferd, Zebrafisch (Danio rerio) und Saccharomyces cerevisiae.

Kurzübersicht für Calreticulin Antikörper (C-Term) (ABIN2779284)

Target: Calreticulin (CALR)

Reaktivität: Human, Ratte, Maus, Hund, Meerschweinchen, Rind (Kuh), Pferd, Zebrafisch (Danio rerio), Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Calreticulin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Calreticulin Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: CALR Antibody - C-terminal region

Sequenz: KEEEEDKKRK EEEEAEDKED DEDKDEDEED EEDKEEDEEE DVPGQAKDEL

Homologie: Cow: 86%, Dog: 92%, Guinea Pig: 93%, Horse: 92%, Human: 100%, Mouse: 93%, Rat: 86%, Yeast: 86%, Zebrafish: 85%

Produktmerkmale: This is a rabbit polyclonal antibody against CALR. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CALR

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8096067

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Details zu Calreticulin

Target: Calreticulin (CALR)

Andere Bezeichnung: CALR

Hintergrund:

Background Information: Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.

Gene Name: Calreticulin

Alternative Symbols: RO, CRT, SSA, cC1qR, HEL-S-99n

Protein Name: Calreticulin

Molekulargewicht: 48kDa

Gen-ID: 811

NCBI Accession: NP_004334

UniProt: P27797

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response