PTBP1 Antikörper (Middle Region)

Produktnummer ABIN2778815

Dieses Anti-PTBP1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PTBP1 in WB. Geeignet für Human, Maus, Ratte, Rind (Kuh), Hund, Meerschweinchen, Pferd, Kaninchen und Zebrafisch (Danio rerio). Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für PTBP1 Antikörper (Middle Region) (ABIN2778815)

Target: PTBP1 (Polypyrimidine Tract Binding Protein 1 (PTBP1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Meerschweinchen, Pferd, Kaninchen, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PTBP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-PTBP1 Antikörper

Bindungsspezifität: Middle Region

Sequenz: KGFKFFQKDR KMALIQMGSV EEAVQALIDL HNHDLGENHH LRVSFSKSTI

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against PTBP1. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PTBP1

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 557 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-PTBP1 Antikörper (ABIN2778815)

Clower, Chatterjee, Wang, Cantley, Vander Heiden, Krainer: "The alternative splicing repressors hnRNP A1/A2 and PTB influence pyruvate kinase isoform expression and cell metabolism." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 5, pp. 1894-9, (2010) (PubMed).

Details zu PTBP1

Target: PTBP1 (Polypyrimidine Tract Binding Protein 1 (PTBP1))

Andere Bezeichnung: PTBP1

Hintergrund: PTBP1 belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described.
Alias Symbols: HNRNP-I, HNRNPI, HNRPI, MGC10830, MGC8461, PTB, PTB-1, PTB-T, PTB2, PTB3, PTB4, pPTB
Protein Interaction Partner: UBC, SNRPA, SFPQ, PTBP1, NONO, LGR4, MDM2, RPA3, RPA2, RPA1, BMI1, SUZ12, EED, RNF2, DIMT1, YBX3, AIMP2, SPTAN1, RPS27, QARS, YBX1, RPSA, ILF2, HNRNPU, HNRNPD, HNRNPA2B1, FAU, EPRS, EIF4G1, APBB1, ABCF1, NELFB, G3BP1, AIMP1, EIF2B2, IQGAP1, TARDBP, SRPK2,
Protein Size: 557

Molekulargewicht: 60 kDa

Gen-ID: 5725

NCBI Accession: NM_002819, NP_002810

UniProt: Q29099

Pathways: Regulation of Muscle Cell Differentiation