Fibrillarin Antikörper (N-Term)

Produktnummer ABIN2778775

Der Kaninchen Polyklonal Anti-Fibrillarin-Antikörper wurde für WB und IHC validiert. Er ist geeignet, Fibrillarin in Proben von Human, Ratte, Maus, Hund, Rind (Kuh), Kaninchen, Meerschweinchen und Pferd zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für Fibrillarin Antikörper (N-Term) (ABIN2778775)

Target: Fibrillarin (FBL)

Reaktivität: Human, Ratte, Maus, Hund, Rind (Kuh), Kaninchen, Meerschweinchen, Pferd

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Fibrillarin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-Fibrillarin Antikörper

Bindungsspezifität: N-Term

Sequenz: GGGFHSGGNR GRGRGGKRGN QSGKNVMVEP HRHEGVFICR GKEDALVTKN

Homologie: Cow: 86%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against FBL. It was validated on Western Blot and immunohistochemistry.

Aufreinigung: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human FBL

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 321 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-Fibrillarin Antikörper (ABIN2778775)

Ernoult-Lange, Wilczynska, Harper, Aigueperse, Dautry, Kress, Weil: "Nucleocytoplasmic traffic of CPEB1 and accumulation in Crm1 nucleolar bodies." in: Molecular biology of the cell, Vol. 20, Issue 1, pp. 176-87, (2009) (PubMed).

Armistead, Khatkar, Meyer, Mark, Patel, Coghlan, Lamont, Liu, Wiechert, Cattini, Koetter, Wrogemann, Greenberg, Entian, Zelinski, Triggs-Raine: "Mutation of a gene essential for ribosome biogenesis, EMG1, causes Bowen-Conradi syndrome." in: American journal of human genetics, Vol. 84, Issue 6, pp. 728-39, (2009) (PubMed).

Details zu Fibrillarin

Target: Fibrillarin (FBL)

Andere Bezeichnung: FBL

Hintergrund: FBL is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. FBL contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8 % of humans with the autoimmune disease scleroderma recognize fibrillarin.This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8 % of humans with the autoimmune disease scleroderma recognize fibrillarin.
Alias Symbols: FIB, FLRN, RNU3IP1
Protein Interaction Partner: UBC, CEP250, TP53, SUMO2, SUMO3, LIN28A, LIN28B, RPA3, RPA2, RPA1, ERG, RNF2, BMI1, SUZ12, EED, EZH2, TARDBP, UBD, NCL, ARFGEF1, PAN2, CBX8, ITGA4, FN1, VCAM1, MAGOH, EIF4A3, EEF1A1, DHX15, NSUN2, NOP58, MRTO4, GNL3, RSL1D1, SRRM2, BOP1, RRS1, EBNA1BP2, P
Protein Size: 321

Molekulargewicht: 35 kDa

Gen-ID: 2091

NCBI Accession: NM_001436, NP_001427

UniProt: P22087

Pathways: Ribonucleoside Biosynthetic Process