Fibrillarin Antikörper

Produktnummer ABIN1580417

Produktdetails anti-Fibrillarin Antikörper

Target: Fibrillarin (FBL)

Reaktivität: Saccharomyces cerevisiae

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Fibrillarin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Aufreinigung: conc. tissue culture supernatant

Klon: 38F3

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: For western blots of yeast protein samples, use MCA-38F3 diluted 1/2,000 (cell lysates) to 1/10,000 (nuclear fractions), followed by chemiluminescent detection (ECL). For other (non-ECL) western detection methods, try MCA-38F3 diluted 1/1,000 to 1/5,000. To detect mammalian fibrillarin on western blots by ECL, try MCA-38F3 at 1/500 dilution. For immunofluorescence on yeast cells, use MCA-38F3 diluted 1/1,000 to 1/5,000. For IF of mammalian cells, try MCA-38F3 at 1/500.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 4°C short term or -20°C long term.

Referenzen für anti-Fibrillarin Antikörper (ABIN1580417)

Grimmer, Bacia: "Giant Endoplasmic Reticulum vesicles (GERVs), a novel model membrane tool." in: Scientific reports, Vol. 10, Issue 1, pp. 3100, (2020) (PubMed).

Details zu Fibrillarin

Target: Fibrillarin (FBL)

Andere Bezeichnung: Nop1p, Fibrillarin (FBL Produkte)

Synonyme: FIB antikoerper, FLRN antikoerper, RNU3IP1 antikoerper, wu:fb37g12 antikoerper, zgc:56145 antikoerper, zgc:77130 antikoerper, FBL antikoerper, AL022665 antikoerper, CG9888 antikoerper, Dmel\\CG9888 antikoerper, Fibri antikoerper, GCR-6 antikoerper, GCR6 antikoerper, Pen59C5 antikoerper, fib antikoerper, pen59C5 antikoerper, MGC76139 antikoerper, fbl antikoerper, fibrillarin antikoerper, Fibrillarin antikoerper, putative fibrillarin antikoerper, fibrillarin S homeolog antikoerper, FBL antikoerper, fbl antikoerper, Fbl antikoerper, Fib antikoerper, fib1 antikoerper, LMJF_19_0100 antikoerper, fbl.S antikoerper

Hintergrund: Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is 327 amino acids in size, is essential for yeast viability, and is localized in the nucleoli. The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA.Nop1p is the yeast homologue of a protein apparently found in all eukaryotes and archea generally called fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. This means that suitably cross-reactive antibodies to Nop1p/fibrillarin, like MCA-38F3, can be used to reveal nucleoli and study fibrillarin/Nop1p in all eukaryotes and archea tested to date. Human fibrillarin has been characterized (accession P22087) and the human fibrillarin gene is located on chromosome 19. Fibrillarin/Nop1p proteins have been cloned and sequenced from several other species (e.g. Mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551. The N terminal approx. 80 amino acids contain multiple copies based on the peptide RGG, or arginine-glycine-glycine, sometimes referred to as GAR repeats, characteristic of the GAR family of molecules. The remaining approx. 240 amino acids consist of the so called fibrillarin domain. A fibrillarin homologue has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-terminal extension but is clearly homologous to the other sequences throughout all of the fibrillarin domain. The 3D structure of this molecule has been determined and shown to consist of 2 extended beta,-sheets flanked by alpha,-helixes (Medline link). Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a approx. 34 kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable. This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6). The HGNC name for this protein is FBL.

Pathways: Ribonucleoside Biosynthetic Process