LIG4 Antikörper (N-Term)

Produktnummer ABIN2778272

Der Kaninchen Polyklonal Anti-LIG4-Antikörper wurde für WB und IHC validiert. Er ist geeignet, LIG4 in Proben von Human, Maus, Ratte, Rind (Kuh), Hund, Pferd, Meerschweinchen und Kaninchen zu detektieren.

Kurzübersicht für LIG4 Antikörper (N-Term) (ABIN2778272)

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Pferd, Meerschweinchen, Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LIG4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-LIG4 Antikörper

Bindungsspezifität: N-Term

Sequenz: DGERMQMHKD GDVYKYFSRN GYNYTDQFGA SPTEGSLTPF IHNAFKADIQ

Homologie: Cow: 92%, Dog: 93%, Guinea Pig: 93%, Horse: 86%, Human: 100%, Mouse: 92%, Rabbit: 93%, Rat: 93%

Produktmerkmale: This is a rabbit polyclonal antibody against LIG4. It was validated on Western Blot and immunohistochemistry.

Aufreinigung: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human LIG4

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 911 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu LIG4

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Andere Bezeichnung: LIG4

Hintergrund: LIG4 encodes a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome.
Protein Interaction Partner: XRCC4, UBC, AGO2, GZMK, ZSCAN18, PHF10, PNKP, TSFM, PRKDC, GUSBP3, NOMO3, FBLL1, PGP, ASPM, AMZ2P1, PPP1R18, COMMD1, ZNF428, AGAP4, WDR20, RPS19BP1, ZFAND2A, FIP1L1, CCDC176, VKORC1, COA7, SOWAHC, SEMA4G, ALMS1, WARS, UBE2L3, UBB, TPT1, TPI1, TP53BP1, TLE
Protein Size: 911

Molekulargewicht: 104 kDa

Gen-ID: 3981

NCBI Accession: NM_002312, NP_002303

UniProt: Q8IY66

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response