MGMT Antikörper (Middle Region)

Produktnummer ABIN2777118

Der Kaninchen Polyklonal anti-MGMT Antikörper (ABIN2777118) detektiert spezifisch MGMT in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte, Maus, Hund, Meerschweinchen, Pferd, Kaninchen und Saccharomyces cerevisiae.

Kurzübersicht für MGMT Antikörper (Middle Region) (ABIN2777118)

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reaktivität: Human, Ratte, Maus, Hund, Meerschweinchen, Pferd, Kaninchen, Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MGMT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-MGMT Antikörper

Bindungsspezifität: Middle Region

Verwendungszweck: MGMT Antibody - middle region

Sequenz: ISYQQLAALA GNPKAARAVG GAMRGNPVPI LIPCHRVVCS SGAVGNYSGG

Homologie: Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 79%

Produktmerkmale: This is a rabbit polyclonal antibody against MGMT. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MGMT

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP42038-100UG

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Details zu MGMT

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Andere Bezeichnung: MGMT

Hintergrund:

Background Information: MGMT is involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA.MGMT repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Gene Name: O-6-methylguanine-DNA methyltransferase

Alternative Symbols: -

Protein Name: Methylated-DNA--protein-cysteine methyltransferase

Molekulargewicht: 22kDa

Gen-ID: 4255

NCBI Accession: NP_002403

UniProt: P16455

Pathways: DNA Reparatur, Positive Regulation of Response to DNA Damage Stimulus