MGMT Antikörper (Middle Region)

Produktnummer ABIN2777118

Der Kaninchen Polyklonal anti-MGMT Antikörper (ABIN2777118) detektiert spezifisch MGMT in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte, Maus, Hund, Meerschweinchen, Pferd, Kaninchen und Saccharomyces cerevisiae.

Kurzübersicht für MGMT Antikörper (Middle Region) (ABIN2777118)

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reaktivität: Human, Ratte, Maus, Hund, Meerschweinchen, Pferd, Kaninchen, Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MGMT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-MGMT Antikörper

Bindungsspezifität: Middle Region

Sequenz: ISYQQLAALA GNPKAARAVG GAMRGNPVPI LIPCHRVVCS SGAVGNYSGG

Homologie: Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 79%

Produktmerkmale: This is a rabbit polyclonal antibody against MGMT. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MGMT

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 207 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu MGMT

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Andere Bezeichnung: MGMT

Hintergrund: MGMT is involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA.MGMT repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Alias Symbols: -
Protein Interaction Partner: UBC, BRCA2, ANXA2, ANXA1, SLC25A6, SLC25A4, ACTB, CCNB1, FBL, EEF1B2, EEF1A2, EEF1A1, DDX5, CDKN1A, CDK2, CDK1, H2BFM, FBXO27, TUBA1C, ANP32E, IL25, DDX24, CDK12, FBXO2, USP19, TUBB4B, PRPF4, RNF8, EIF3H, CDK10, HIST1H4A, HIST1H2AM, HIST3H3, SMC1A, YWHAZ,
Protein Size: 207

Molekulargewicht: 22 kDa

Gen-ID: 4255

NCBI Accession: NM_002412, NP_002403

UniProt: P16455

Pathways: DNA Reparatur, Positive Regulation of Response to DNA Damage Stimulus